Differential expression of isocitrate lyase in P. marneffei phagocytized by nonstimulated and stimulated murine macrophages.
- Author:
Jun LI
1
;
Li-yan XI
;
Hong-fang LIU
;
Jun-min ZHANG
;
Xi-qing LI
;
Xiao-rong XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Fungal Proteins; genetics; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; drug effects; Gene Expression Regulation, Fungal; drug effects; Host-Pathogen Interactions; Interferon-gamma; pharmacology; Isocitrate Lyase; genetics; Lipopolysaccharides; pharmacology; Macrophages; drug effects; immunology; microbiology; Mice; Nitric Oxide; immunology; Penicillium; genetics; immunology; physiology; Phagocytosis; immunology; Reverse Transcriptase Polymerase Chain Reaction; omega-N-Methylarginine; pharmacology
- From: Journal of Southern Medical University 2007;27(5):631-633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential expression of isocitrate lyase in Penicillium marneffei phagocytized by nonstimulated and stimulated murine macrophages, and explore the role of glyoxylate pathway in pathogenesis of Penicilliosis marneffei.
METHODSPenicillium marneffei conidia and Raw264.7 cells were incubated in 16 cultures, which were divided to 4 groups for treatment with N-monomethyl-L-arginine (LNMMA, CI group), murine interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS) (T group), IFN-gamma plus LPS and LNMMA (TI group), or the same volume of culture medium (C group). The transcriptional levels of isocitrate lyase were detected using real-time RT-PCR, and its expression levels detected biochemically.
RESULTSThe transcriptional levels of isocitrate lyase in C, CI, T, TI groups were 1.00, 1.42, 33.09, and 74.88 (P<0.05), while the expression levels were 0.06, 0.07, 0.18, and 0.93, respectively (P<0.05). The content of nitric oxide in T group was significantly higher than that in the other groups (P<0.01), but the CFU of T group was the lowest (P<0.01).
CONCLUSIONReactive nitrogen intermediates induced by stimulated murine macrophages restrain the expression of isocitrate lyase of Penicillium marneffei and development of Penicillium marneffei, in which process the glyoxylate pathway may play an important role.
