Bulky DNA adducts in human sperm correlate with semen parameters and sperm apoptosis.
- Author:
Gui-Xiang JI
1
;
Sheng-Min WU
;
Ji-Ning LIU
;
Li-Li SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Apoptosis; DNA Adducts; analysis; Humans; Infertility, Male; pathology; physiopathology; Male; Middle Aged; Polycyclic Aromatic Hydrocarbons; analysis; Semen; chemistry; Semen Analysis; Sperm Count; Spermatozoa; Young Adult
- From: National Journal of Andrology 2013;19(10):902-906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the correlation of sperm polycyclic aromatic hydrocarbons (PAH)-DNA adducts with semen quality and sperm apoptosis.
METHODSWe collected semen samples from 433 infertile Chinese men, detected sperm PAH-DNA adducts using immunofluorescence staining and flow cytometry, and determined the rate of sperm apoptosis by TUNEL.
RESULTSMultiple linear regression analysis showed that the level of sperm PAH-DNA adducts was correlated negatively with sperm concentration (beta = -0.632), total sperm count (beta = -0.830) and sperm motility (beta = -9.647), but positively with the rate of sperm apoptosis (beta = 0.130).
CONCLUSIONSperm PAH-DNA adducts are evidently correlated with semen quality and sperm apoptosis, and play an important role in the evaluation of male productivity.
