Expression of insulin-like factor 3 in the testis of flutamide-induced cryptorchidism mice and its significance.
- Author:
Yao-Mei YUAN
1
;
Ci ZHANG
2
;
Chen BAI
2
;
Ming ZHENG
2
;
Xiang-Jun ZHOU
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cryptorchidism; chemically induced; metabolism; Female; Flutamide; toxicity; Insulin; metabolism; Male; Maternal Exposure; adverse effects; Mice; Mice, Inbred BALB C; Pregnancy; Proteins; metabolism; RNA, Messenger; genetics; Testis; metabolism
- From: National Journal of Andrology 2013;19(11):968-971
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the changes in the mRNA expression of insulin-like factor 3 (INSL-3) in the testis of mouse models of flutamide-induced cryptorchidism.
METHODSWe randomized pregnant BALB/c mice to groups A (control) , B, C, D and E to receive continuous gavage of flutamide at 0, 150, 300, 500 and 700 mg/kg body weight, respectively, from gestation day 12 to 21. We detected the expression of INSL-3 mRNA in the testis of the neonates by real-time PCR at 4 and 8 postnatal weeks.
RESULTSNo cryptorchidism was found in group A; unilateral cryptorchidism was seen in groups B (10.0%) and C (25.0%); and bilateral cryptorchidism was observed in groups D (21.1%) and E (40.0%). The expression of INSL-3 mRNA was reduced with the increased dose of flutamide, not significantly changed in groups B and C (P > 0.05) but remarkably decreased in D and E as compared with A (P < 0.05).
CONCLUSIONAdministration of flutamide to pregnant mice can induce unilateral cryptorchidism at 150 and 300 mg/kg and bilateral cryptorchidism at 500 and 700 mg/kg in their male offspring, which may be related with its reducing effect on the expression of INSL-3 in the testis of the mice.
