Expressions of netrin-1 and UNC5B in prostate cancer and their clinical significance.
- Author:
Jiao LIU
1
;
Chui-Ze KONG
1
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; metabolism; Cell Line, Tumor; Humans; Male; Nerve Growth Factors; metabolism; Netrin-1; Prostatic Neoplasms; metabolism; pathology; Receptors, Cell Surface; metabolism; Tumor Suppressor Proteins; metabolism
- From: National Journal of Andrology 2013;19(12):1072-1076
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for a new diagnostic biomarker for prostate cancer by comparing the differences in the expressions of netrin-1 and UNC5B in prostate cancer cells with different invasive abilities.
METHODSWe examined the expressions of netrin-1 and UNC5B in five prostate cancer cell lines DU145, 22RV1, PC3, PC3M and RWPE-1 using RT-PCR and Western blot, and positioned the ligands netrin-1 and its receptor UNC5B in the prostate cancer cells by immunofluorescence.
RESULTSBoth netrin-1 and UNC5B were expressed in the prostate cancer cells, and the expression of netrin-1 was significantly increased in highly invasive cells (P < 0.05), while that of UNC5B in RWPE-1 (normal) cells (P < 0.05).
CONCLUSIONThe expressions of netrin-1 and UNC5B are closely related to the infiltration and progression of prostate cancer, and expected to be as potential biomarkers for predicting the malignancy degree of prostate cancer.
