Ex vivo expansion of CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria and their hematopoietic reconstitution capability in irradiated nude mice.
- Author:
Yu-Ping ZHONG
1
;
Yong-Ji WU
;
Ti SHEN
;
Xuan WANG
;
Jie-Ping ZHANG
Author Information
1. Department of Hematology, Beijing Chaoyang Hospital (Jingxi Campus), Capital University of Medical Sciences Bejing 100043, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigens, CD34;
analysis;
Bone Marrow Cells;
cytology;
immunology;
CD59 Antigens;
analysis;
Female;
Hematopoiesis;
physiology;
Hematopoietic Stem Cell Transplantation;
Hemoglobinuria, Paroxysmal;
pathology;
Humans;
Immunomagnetic Separation;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Transplantation, Heterologous;
Whole-Body Irradiation
- From:
Journal of Experimental Hematology
2008;16(3):561-564
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the expansion and hematopoietic reconstitution capability of CD34(+)CD59(+) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH) by using BALB/c nude mice so as to provide experimental basis for clinical anto-BMT or auto-PBHSCT in patients with PNH. CD34(+)CD59(+) cells were selected from the bone marrow mononuclear cells in normal persons and PNH patients by immunomagnetic positive double sorting and were engrafted sublethally irradiated BALB/c nude mice. The human CD45(+) cells in bone marrow, spleen and peripheral blood of recipient mice were detected by flow cytometry and DNA assay. The results showed that the CD34(+)CD59(+) cells in PNH patient group and normal person group could expanded ex vivo, but ex vivo expansion capability of CD34(+)CD59(+) cells in PNH patient group at day 7 seemed inferior to that in normal control. While CD34(+)CD59(+) cells of PNH patients and normal persons were transfused into recipient mice, the human CD45(+) cells could be detected in bone marrow, spleen and peripheral blood at 6 weeks after transfusion, but there was no statistical difference in counts of CD45 cells between 2 groups. It is concluded that CD34(+)CD59(+) cells from PNH patients may keep characteristics of normal hematopoietic stem cells, and possess ability to expand ex vivo and support hemopoiesis.
