Activation of specific T lymphocyte induced by artificial antigen presenting cell complex in vitro.
- Author:
Han-Wen MAO
1
;
Wen-Li LIU
;
Jian-Feng ZHOU
;
Zhe GENG
;
Wei HUANG
Author Information
1. Deparment of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. MHW555@21CN.COM
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antigen Presentation;
immunology;
Antigen-Presenting Cells;
immunology;
metabolism;
Antigens, Neoplasm;
immunology;
Biomimetics;
methods;
CD8-Positive T-Lymphocytes;
immunology;
Female;
HLA-A2 Antigen;
immunology;
Humans;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
immunology;
Lymphocyte Activation;
Male;
Middle Aged;
Tissue Donors;
Young Adult
- From:
Journal of Experimental Hematology
2008;16(3):655-658
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of artificial antigen-presenting cells (aAPCs) on inducing activation and proliferation of specific T-lymphocytes through stimulating biological function of dendritic cells with aAPCs in vitro. The specific antigen of chronic myeloid leukemia CML-28 was screened as objective antigen peptide by using magnetic microbeads as vector; the CML-28 epitope sequence (Vltfaldsv) was obtained by antigen epitope prediction software; this epitope was coupled with MHC molecule and used as first signal molecule, the B7-1 molecule was used as second signal molecule; these 2 molecules simultaneously were loaded onto surface of magnetic microbeads so as to contract aAPC complex. The bone marrow mononuclear cells were derived from HLA-A2(+) healthy bone marrow donors, CD8(+) T lymphocytes were screened and co-cultured with aAPC complex. During culture the 5, 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) was added and proliferation of T-lymphocytes was detected by CPSE and proliferation level of specific T lymphocytes was detected by flow cytometry. The results showed that the proliferation level of CML-28 specific T lymphocytes obviously increased in experimental group, average level was 17.34 +/- 0.65%, while average level in control was 2.25 +/- 0.43%, there was significant difference between them (p < 0.01). It is concluded that the aAPC complex can mimic human APCs in vitro, and stimulate activation and proliferation of CD8(+) specific T lymphocytes.
