BRAF-Activated Long Noncoding RNA Modulates Papillary Thyroid Carcinoma Cell Proliferation through Regulating Thyroid Stimulating Hormone Receptor.
- Author:
Haitao ZHENG
1
;
Meng WANG
;
Lixin JIANG
;
Haidi CHU
;
Jinchen HU
;
Jinyao NING
;
Baoyuan LI
;
Dong WANG
;
Jie XU
Author Information
- Publication Type:Original Article
- Keywords: Long noncoding RNA; Thyroid neoplasms; BRAF-activated long noncoding RNA
- MeSH: Carcinogenesis; Cell Cycle Checkpoints; Cell Line; Cell Proliferation*; Chromatin; Cyclin D1; Down-Regulation; Polymerase Chain Reaction; Protein Kinases; Receptors, Thyrotropin*; Reverse Transcription; RNA, Long Noncoding*; RNA, Untranslated; Thyroid Gland*; Thyroid Neoplasms*; Thyrotropin*
- From:Cancer Research and Treatment 2016;48(2):698-707
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: The importance of long noncoding RNAs (lncRNAs) in tumorigenesis has recently been demonstrated. However, the role of lncRNAs in development of thyroid cancer remains largely unknown. MATERIALS AND METHODS: Using quantitative reverse transcription polymerase chain reaction, expression of three lncRNAs, including BRAF-activated long noncoding RNA (BANCR), papillary thyroid cancer susceptibility candidate 3 (PTCSC3), and noncoding RNA associated with mitogen-activated protein kinase pathway and growth arrest (NAMA), was investigated in the current study. RESULTS: Of the three lncRNAs (BANCR, PTCSC3, and NAMA), expression of BANCR was significantly up-regulated while PTCSC3 and NAMA were significantly down-regulated in papillary thyroid carcinoma (PTC) compared to that in normal tissue. BANCR-knockdown in a PTC-derived cell line (IHH-4) resulted in significant suppression of thyroid stimulating hormone receptor (TSHR). BANCR-knockdown also led to inhibition of cell growth and cell cycle arrest at G0/G1 phase through down-regulation of cyclin D1. In addition, BANCR was enriched by polycomb enhancer of zeste homolog 2 (EZH2), and silencing BANCR led to decreased chromatin recruitment of EZH2, which resulted significantly reduced expression of TSHR. CONCLUSION: These findings indicate that BANCR may contribute to the tumorigenesis of PTC through regulation of cyclin D1 and TSHR.
