Gene expression profile of human TGF-beta signal transduction pathway of B cell type acute lymphocytic leukemia.
- Author:
She-Dan LUO
1
;
Yong WU
;
Jian-Hua CHEN
;
He-Kun LIU
;
Yuan-Zhong CHEN
Author Information
1. Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian Province, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Gene Expression Profiling;
Humans;
Leukemia, B-Cell;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Signal Transduction;
genetics;
Transforming Growth Factor beta;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2008;16(4):742-745
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the gene expression profile of TGF-beta signal transduction pathway in B-cell type acute lymphocytic leukemia (B-ALL). The gene expression profiles in B-ALL primary cells and cell lines (NALM6 cells, Raji cells), B-lymphocyte of control were detected by cDNA microarray including 113 different genes in human TGF-beta/BMP signal transduction pathway, and TGF-beta(1) mRNA expression was detected by real time RT-PCR. The B lymphocytes in peripheral blood of heacthy persons sorted by flow cytometry were used as control. The difference between them was compared. The results showed that as compared with B lymphocytes in peripheral blood of heacthy persons, the TGF-beta(1) expression in B-ALL cells, NALM6 cells and Raji cells were down-regulationed, myc and smad1 gene expressions were up-regulated, IL-6, smad 7 gene expressions were down-regulated. It is concluded that TGF-beta signal transduction is abnormal in B-cell type acute lymphocytic leukemia.
