Effects of some traditional Chinese drugs on Mdr1 gene and its expression product in K562/A02 cells.
- Author:
Na GAO
1
;
Yu ZHANG
;
Jun-Qing MAO
;
Guo-Qing LI
;
Wei ZHOU
;
Bo GAO
;
Jian GU
Author Information
1. Departmant of Hematology and Rheumatology, Subei People Hospital of Jiangsu Province, Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
genetics;
metabolism;
Down-Regulation;
drug effects;
Doxorubicin;
pharmacology;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Drugs, Chinese Herbal;
pharmacology;
Humans;
K562 Cells;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2008;16(4):785-789
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effects of angelicasinensis (Oliv) Diel compound injection, vauqueline, ephedrine and strychnine on human erythroleukemia multidrug resistance (MDR) K562/A02 cell line Mdr1 gene and p-glycoprotein. The MTT and trypan blue methods were used to analyze the cytotoxic effect of above-mentioned traditional Chinese drug; the expressions of K562/A02 cells Mdr1 gene and p-glycoprotein were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively. The results showed that after K562/A02 cell were treated with angelicasinensis (Oliv) diels compound injection, vauqueline and ephedrine, mRNA transcription of Mdr1 gene was reduced significantly (p < 0.01); the expression of P-gp also decreased (p < 0.01). The expression level P-gp in group treated with vauqueline was the lowest, but the Mdr1 mRNA level and expression of P-gp of K562/A02 cells treated with strychnine did not obviously changed. It is concluded that angelicasinensis (Oliv) diels compound injection, vauqueline, ephedrine can partly reverse the multidrug resistance of K562/A02 cells, the down-regulation of Mdr1 gene causing decrease of p-glycoprotein expression may be one of the MDR reversal mechanisms in K562/A02 cells.
