Analysis of STR loci expression after allogeneic cord blood hematopoietic stem cell transplantation.
- Author:
Xin YE
1
;
Wen-Jie XIA
;
Lu-Lu XIAO
;
Hao-Qiang DING
;
Xia RONG
Author Information
1. Guangzhou Tissue and Organ Typing Center, Guangzhou 510095, Guangdong Province, China.
- Publication Type:Case Reports
- MeSH:
Child;
Cord Blood Stem Cell Transplantation;
Genetic Loci;
genetics;
Humans;
Male;
Microsatellite Repeats;
genetics;
Muscular Dystrophies;
genetics;
therapy;
Transplantation Chimera;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2008;16(4):843-846
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to analyze the STR loci expression after allergenic cord hematopoietic stem cell transplantation in patient with Ducennes muscular dystropy (DMD) patient. PCR-SSO was used to identify the HLA antigens and alleles, STR-PCR was used to detect the chimera status. Quantity analysis of donor chimeras was performed by multiplex PCR amplification of STR marker and capillary electrophoresis with fluorescence detection. The results showed that patient appear to be HLA identical to the donor cord blood at the tested level. Persistent full donor chimerism was found in breast bone marrow. The patient with stable MC (DC < 5%) had a probability of long term survival with molecular remission MC status appeared in forearm muscle, tongue, liver, spleen, stomach, right temporal lobe, diaphragmatic muscle, bronchus, left ventricle and right kidney. In conclusion, the donor gene can express in parenchymatoas organs, the donor chimerism was detected in breast bone marrow and some other organs.
