Application of thrombelastography in evaluation of platelet function during storage.
- Author:
Yang YU
1
;
Zi-Lin LIN
;
Qian FEN
;
Ji-Chun PAN
;
Ting ZHANG
;
Gui-Xiang SUN
;
Xiao-Juan ZHANG
;
Chun-Ya MA
;
Guo-Feng GE
;
De-Qing WANG
;
Qun LUO
;
Ya-Ping TIAN
Author Information
1. Department of Blood Transfusion, Center for Clinical Transfusion Medicine, PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
physiology;
Blood Preservation;
Humans;
Platelet Function Tests;
methods;
Thrombelastography
- From:
Journal of Experimental Hematology
2008;16(4):926-929
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore changes of platelet function in vitro during storage by thrombelastography (TEG). 12 units plateletpheresis were randomly selected and stored at 20 to 24 degrees C with agitation. Thrombelastography variable parameters R, K values and maximal amplitude (MA) were measured on 1, 2, 3, 4, 5 days of platelet storage. Platelet concentration, mean platelet volume (MPV), hypotonic shock response (HSR), CD62p expression and CD62p reexpression on platelet surface were detected at the same time. Changes of platelet function in virto were systematically evaluated by above-mentioned indexes. The results showed that MPV augmented slightly with prolongation of preserved time (p > 0.05), and CD62p expression on platelet surface increased remarkably (p < 0.01), while CD62p reexpression decreased gradually (p < 0.01). There were no significant differences in HSR level of platelets during storage (p > 0.05). R value increased with prolongation of preserved time (p < 0.01). There were no obvious changes on K value and alpha Angle during storage (p > 0.05). There were no obvious changes in MA from 1 to 4 days, and MA decreased slightly on day 5 (p < 0.05). It is concluded that there was no significant change in MA and HSR which reflects comprehensive coagulation of platelets during storage. Platelets on the end of storage have excellent function of hemostasis; Thrombelastography parameter MA value can be used as a valuable indicator for evaluation of platelet function in vitro during storage.
