Expression and clinical significance of antiapoptosis gene XIAP in prostate cancer.
- Author:
Jianhe LIU
1
;
Ming LI
;
Shujie XIA
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Aged, 80 and over; Cell Line, Tumor; Humans; Immunohistochemistry; Male; Neoplasm Staging; Prostatic Neoplasms; metabolism; pathology; Reverse Transcriptase Polymerase Chain Reaction; X-Linked Inhibitor of Apoptosis Protein; biosynthesis; genetics
- From: National Journal of Andrology 2004;10(11):832-835
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of the inhibitor of apoptosis (IAP) gene XIAP in prostate cancer and the relationship between its expression and its clinical stage or Gleason grade.
METHODSXIAP mRNA expression was detected by RT-PCR in three prostate cancer cell lines (PC-3, DU-145, LNCaP), neoplastic prostate tissues and normal prostate tissues. Immunohistochemical SP method was used to examine the expression of XIAP protein in 56 neoplastic prostate tissue specimens.
RESULTSXIAP gene was not expressed in normal prostate tissues, but highly expressed in prostate cancer cell lines PC-3, DU-145, LNCaP. Thirty of the 56 (53.6%) tumor samples were positive XIAP protein, and only 12 (21.5%) paratumor samples were positive XIAP protein (P < 0.01), XIAP positivity not correlated with tumor stage or Gleason grade (P > 0.05).
CONCLUSIONXIAP may be involved in the development of prostate cancer and play an important role in human prostate carcinogenesis. It is likely to be used as a target of prostate cancer therapy.
