Segregation of sex chromosomes in the spermatozoa of 46, XY/47, XXY patients with oligozoospermia by dual fluorescence in-situ hybridization.
- Author:
Yingxia CUI
1
;
Yifeng GE
;
Yunhua WANG
;
Qin ZHOU
;
Yongmei WANG
;
Peiyuan ZHU
;
Xuejun SHANG
;
Yufeng HUANG
Author Information
- Publication Type:Case Reports
- MeSH: Adult; Chromosomes, Human, X; Chromosomes, Human, Y; Humans; In Situ Hybridization, Fluorescence; Klinefelter Syndrome; genetics; therapy; Male; Oligospermia; genetics; therapy; Sperm Injections, Intracytoplasmic
- From: National Journal of Andrology 2004;10(11):841-843
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the segregation of sex chromosomes in the spermatozoa of a 46, XY/47, XXY patient with oligozoospermia.
METHODSThe number of X and Y chromosomes of the ejaculated spermatozoa from the patient with mosaic 46, XY/47, XXY was analysed by X/Y dual fluorescence in-situ hybridization (FISH).
RESULTSOf the 100 spermatozoa analysed, 97 showed either one X chromosome-specific green signal or one Y-chromosome-specific red Y signal in each spermatozoon and only 3 showed no signal. The frequencies of X- and Y-bearing spermatozoa were 49% and 48% respectively. The ratio of X- to Y-bearing spermatozoa was about 1:1 as expected. There was no statistical difference between the chromosome XX and XY frequencies in each spermatozoon from the patient in comparison with those estimated in the control.
CONCLUSIONThe spermatozoa of 46, XX/47, XXY mosaic patients have a normal gonosomal complement, which allows infertility treatment to be carried out by ICSI.
