Experimental study of the biological activities of several alpha1-adrenoceptor antagonists in vitro.
- Author:
Bin WU
1
;
Yan ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenergic alpha-1 Receptor Antagonists; Adrenergic alpha-Antagonists; pharmacology; Anal Canal; drug effects; Animals; In Vitro Techniques; Male; Muscle Contraction; drug effects; Rats; Rats, Sprague-Dawley
- From: National Journal of Andrology 2004;10(12):909-911
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the biological activities of several alpha1-adrenoceptor antagonists in vitro.
METHODSThe anococcygeal muscle from male Sprague-Dawley rats (300-350 g) was isolated, removed and suspended in a 20-ml organ chamber containing Krebs solution at 37 degrees C, pH 7.4. The muscle preparations were set at a resting tension of 1.0 g and allowed to equilibrate for 1 h in the Krebs solution. After thorough washing, the anococcygeal muscle preparations were examined for the effects of the tested compounds with increased concentrations on its contractile/relaxant responses and the pA2 of antagonistic activity was assessed.
RESULTSSome of the target compounds displayed blocking activity to alpha1-adrenoceptor.
CONCLUSIONCompounds WB IV-1 and WB IV-3 showed good inhibiting activity, and were worth further studying.