Effect of MT1M gene on the cell cycle and signaling pathway of Hep-G2.
- Author:
Luhong SUN
1
;
Xiran ZHANG
;
Yahui KONG
;
Long YU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Flow Cytometry; G2 Phase; physiology; Humans; Liver Neoplasms; pathology; Metallothionein; genetics; NF-kappa B; metabolism; Plasmids; genetics; Signal Transduction; physiology; Transfection
- From: National Journal of Andrology 2004;10(12):932-934
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possible roles of MT1M gene on the cell cycle and signaling pathway of Hep-G2.
METHODSHep-G2 human hepatoma cells made by transfection with expressible MT1M gene, and the cell cycle was detected by flow cytometry, and the signaling pathway was measured by dual luciferase assay in Hep-G2 cells.
RESULTSMT1M gene was able to induce changes of the cell cycle and the activation of NF-kappaB pathway in Hep-G2 cells.
CONCLUSIONMT1M gene may affect the cell cycle in Hep-G2 and activate the NF-kappaB-dependent transcription.
