Knockdown of STAT3 expression using siRNA inhibits the growth of prostate cancer cell lines.
- Author:
Li-fang GAO
1
;
De-qi XU
;
Yue-ting SHAO
;
Dan ZHAO
;
Xue-jian ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Humans; Male; Plasmids; Prostatic Neoplasms; metabolism; pathology; RNA, Messenger; genetics; RNA, Small Interfering; STAT3 Transcription Factor; biosynthesis; genetics; Transcription, Genetic
- From: National Journal of Andrology 2005;11(1):29-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of pSilencer 1.0-U6-siRNA-STAT3 on the growth of PC3 and LNCaP cells.
METHODSThree pairs of DNA template coding siRNA were synthesized against STAT3 to reconstruct pSilencer 1.0-U6-STAT3-siRNA, which was transfected into PC3 and LNCaP cells. The STAT3 expression in PC3 cells and LNCaP were transfected with pSilencer 1.0-U6-siRNA-STAT3, and it was detected by Western blot and Northern blot. MTT and FCM were used to observe the growth-inhibiting ratio and apoptosis in PC3 cells.
RESULTSWestern blot and Northern blot analyses demonstrated that pSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit the expression of STAT3 in PC3 and LNCaP cells; MIT and FCM results showed that it could suppress the growth of PC3 cells and LNCaP and induce apoptosis of PC3 cells in vitro.
CONCLUSIONPSilencer 1.0-U6-siRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of PC3 and LNCaP cells and induce the apoptosis of PC3 cells.
