Effect of benzene on sperm DNA.
- Author:
Bo SONG
1
;
Zhi-ming CAI
;
Xin LI
;
Li-xia DENG
;
Lü-kang ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Air Pollutants, Occupational; analysis; Benzene; analysis; toxicity; Comet Assay; DNA Damage; drug effects; Humans; Male; Occupational Exposure; Spermatozoa; drug effects; Surveys and Questionnaires
- From: National Journal of Andrology 2005;11(1):53-55
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the effect of benzene on sperm DNA.
METHODSTwenty-seven workers exposed to benzene for over two years were included in the experiment group and 35 unexposed ones were chosen as controls. Damage by benzene on sperm DNA was detected by modified single cell gel electrophoresis (SCGE).
RESULTSThe benzene concentration in the air of the workplace of the exposed workers was (86.49 +/- 2.83) mg/m3, twice that of the national maximum allowance (40 mg/cm3). Urinary, trans-muconic acid in the exposed group was (1.40 +/- 0.62) mg/L (n = 27), significantly higher than that of the controls [(0.82) +/- 0.16) mg/L, n = 35]. After SCGE, the percentage of head DNA in the exposed group [(70.18% +/- 7.36%, n = 13 ] was significantly lower than that of the controls (90.62% +/- 2.94%, n = 16), P < 0.001.
CONCLUSIONHigher concentration of benzene could cause damage to the sperm DNA of the workers exposed to it.
