Expression of cyclooxygenase-2 in the testes and epididymides of adult male rats.

Author: Xiao-Ni HOU ¹ ; Han-Wang ZHANG ; Yu-Feng LI
Author Information

1. Center of Reproductive Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Publication Type:Journal Article
MeSH: Animals; Cyclooxygenase 2; biosynthesis; Epididymis; enzymology; Immunohistochemistry; Male; Rats; Rats, Sprague-Dawley; Sensitivity and Specificity; Testis; enzymology
From: National Journal of Andrology 2005;11(2):104-111
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the expression of cyclooxygenase-2 (cox-2) in the testes and epididymides of adult male rats and its significance.
METHODSImmunohistochemical staining was used to detect the expression and localization of cox-2 in the testicle and epididymal tissues of 40 adult male SD rats.
RESULTSStrong cox-2 immunoreactivity was detected in the epididymides and testes of the rats. In the caput epididymides, cox-2 expressed mainly in the epithelial nuclei and partly in the cytoplasm. Cox-2 was also found positive in the testis nuclei and cytoplasm.
CONCLUSIONImmunohistochemical staining is a fairly sensitive method for detecting cox-2 expression in the testes and epididymides of adult male rats.