High-performance liquid chromatography for determination of psoralene, bergapten and apigenin in Ficus hirta Vahl.
- Author:
Yan-jun YANG
1
;
Jun DAI
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Ficusin; analysis; Methoxsalen; analogs & derivatives; analysis
- From: Journal of Southern Medical University 2010;30(11):2565-2567
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a high-performance liquid chromatography (HPLC)-based method for determining the contents of psoralene, bergapten and apigenin in Ficus hirta Vahl.
METHODSA Hypersil C18 column (250 mm × 4.6 mm, 5 µm) was used with the mobile phase of methanol-water (60:40), flow rate of 1.0 ml/min, detection wavelength of 268.7 nm and column temperature of 30 degrees celsius.
RESULTSThe calibration curve was linear within the range of 10.0-30.0 µg/ml for psoralene (r=0.9998), 15.0-45.0 µg/ml for bergapten (r=0.9998) and 5.0-15.0 µg/ml for apigenin (r=0.9992). The average recovery of psoralene was 99.7% (RSD=1.99%), that of bergapten was 99.9% (RSD=1.71%) and that of apigenin was 100.3% (RSD=1.78%).
CONCLUSIONThe method is simple, economic and accurate with good reproducibility for the contents of psoralene, bergapten and apigenin.
