Autophagy is involved in 6-OHDA-induced dopaminergic cell death.
- Author:
Li-ping XIA
1
;
Ling-yun LI
;
Xi-feng FEI
;
Zhong-qin LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Autophagy; drug effects; Cell Death; drug effects; Disease Models, Animal; Dopaminergic Neurons; cytology; drug effects; Male; Microtubule-Associated Proteins; metabolism; Oxidopamine; pharmacology; Parkinson Disease, Secondary; chemically induced; metabolism; Phagosomes; metabolism; Rats; Rats, Sprague-Dawley; Substantia Nigra; drug effects
- From: Journal of Southern Medical University 2010;30(12):2649-2651
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of autophagy in the death of dopaminergic neurons induced by 6-hydroxydopamine (6-OHDA).
METHODSRat models of Parkinson disease (PD) were established by stereotaxic administration of 6-OHDA (8 μg) into the unilateral substantia nigra par compact (SNpc). Autophagosomes in the SNpc were observed with transmission electron microscopy (TEM), and the expression of autophagy-related protein LC3 was determined with immunofluorescence (IF) assay.
RESULTSUnder TEM, the autophagosomes were found in the ipsilateral SNpc 6-24 h after 6-OHDA injection, which suggested the activation of autophagy. IF assay showed significantly increased LC3 expression in 6-OHDA-damaged TH-positive neurons as compared to the control group.
CONCLUSIONSThe increase of autophagosomes and activation of autophagy may play a role in dopaminergic neuron death induced by 6-OHDA.
