Differential expression of hsa-miR-126 and hsa-miR-518b in esophageal squamous carcinoma.
- Author:
Hui CAI
1
;
Jian-sheng WANG
;
Ming-xin ZHANG
;
Xiao-yi DUAN
;
Ren-qiang MA
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; metabolism; Carcinoma, Squamous Cell; genetics; pathology; Esophageal Neoplasms; genetics; pathology; Gene Expression Profiling; methods; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; genetics; Tumor Cells, Cultured
- From: Journal of Southern Medical University 2011;31(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the differentially expressed microRNAs (miRNAs) between esophageal squamous carcinoma (ESC) and adjacent non-tumorous tissue (NT).
METHODSThe expression levels of the miRNAs were detected in 3 fresh ESC and NT samples by hybridization with miRNAs microarray chip. Real-time quantitative RT-PCR was performed to confirm the results of the microarray analysis. The expressions of hsa-miR-126 and hsa-miR-518b in ESC were validated by real-time quantitative RT-PCR in another independent 15 matched samples.
RESULTSA total of 11 miRNAs exhibited differential expressions in ESC samples as compared to their expressions in the NT samples, including a 1 up-regulated miRNA and 10 down-regulated miRNAs. Compared with normal esophageal samples, the ESC tissues showed up-regulated hsa-miR-126 and down-regulated hsa-miR-518b expression.
CONCLUSIONhsa-miR-126 and hsa-miR-518b are differentially expressed in ESC, and they might play important roles in the carcinogenesis and progression of ESC.
