Iodoacetamide-induced aquaporin 1 expression in fibroblasts is energy-dependent.
- Author:
Er-ran LI
1
;
Xin HONG
;
Xia LIU
;
Jun-yi SHANG
;
Bo WANG
;
Kun WANG
;
Qiu-yue WANG
;
Jian KANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aquaporin 1; genetics; metabolism; Cells, Cultured; Energy Metabolism; Fibroblasts; cytology; metabolism; Iodoacetamide; pharmacology; Mice; Mice, Inbred BALB C; Mitochondria; metabolism; RNA, Messenger; genetics; metabolism; Up-Regulation; drug effects
- From: Journal of Southern Medical University 2011;31(1):28-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).
METHODSBalb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h.
RESULTSIA induced the expression of AQP1 at 4 and 6 h accompanied with cell death. Reverse transcription PCR showed an increased expression of AQP1 mRNA in the cells. AQP1 expression was also upregulated by the inhibitor of microtubule and cytochrome C oxidase.
CONCLUSIONA pretranslational regulation occurs in IA-induced AQP1 expression in mouse fibroblasts, and the up-regulated AQP1 accumulation is characterized by mitochondria-related energy dependence.
