Radiation-induced G2 phase arrest may contribute to the radioresistance of breast cancer stem cells.
- Author:
Yun-hong TIAN
1
;
Guo-zhu XIE
;
Chen REN
;
Quan-quan SUN
;
Ai-min SUN
;
Ying LIU
;
Ya-wei YUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Breast Neoplasms; pathology; Cell Culture Techniques; methods; Cell Line, Tumor; radiation effects; Female; G2 Phase Cell Cycle Checkpoints; radiation effects; Humans; Mice; Mice, Inbred NOD; Mice, SCID; Neoplastic Stem Cells; pathology; radiation effects; Radiation Tolerance
- From: Journal of Southern Medical University 2011;31(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate radiation-induced cell cycle changes of human breast cancer stem cells enriched by suspension culture.
METHODSThe tumorigenicity of human breast cancer stem cell line MCF-7 cultured in serum-free media was confirmed in NOD/SCID mice, and the radiosensitivity of the cells was tested by clone formation assay following radiation exposure. Flow cytometry was performed to evaluate radiation-induced cell cycle changes, and the protein expression of pCDC25C (ser216) was measured by Western blotting.
RESULTSAfter the exposure to 2 Gy radiation, the survived fraction of the cells in suspension culture and those in adherent culture was 0.856 ∓ 0.061 and 0.783 ∓ 0.097, respectively, and the cells in suspension culture showed an obviously greater capacity of tumorigenicity in NOD/SCID mice. The radiation exposure resulted in an obvious increase in the proportion of G2 phase cells from (22.03 ∓ 2.12)% to (45.83 ∓ 2.25)% and significantly increased the expression of pCDC25C (ser216).
CONCLUSIONRadiation- induced G2 phase arrest may contribute to the resistance of the breast cancer stem cells to radiotherapy.
