Effect of Bushenhuoxue Yin on cerebral levels of nitric oxide, tumor necrosis factor-α and interferon-γ in a mouse model of Parkinson disease.
- Author:
Shao-dan LI
1
;
Yi LIU
;
Ming-hui YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; therapeutic use; Interferon-gamma; metabolism; Male; Mice; Mice, Inbred C57BL; Nitric Oxide; metabolism; Parkinson Disease; drug therapy; metabolism; Phytotherapy; Random Allocation; Tumor Necrosis Factor-alpha; metabolism
- From: Journal of Southern Medical University 2011;31(1):90-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Bushenhuoxue Yin (BSHXY), a compound traditional Chinese herbal medicine, on nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) levels in the brain of a mouse model of Parkinson disease (PD).
METHODSForty-five C57BL/6 mice were randomized into MPTP-induced PD model group, BSHXY treatment group and normal control group. The contents of NO and the two cytokines in the brain tissue of the mice were determined by spectroscopy and enzyme-linked immunosorbent assay, respectively.
RESULTSThe concentration of NO in the brain tissue of the PD model group was 5.93 ∓ 0.79 µmol/g.protein, significantly higher than that in BSHXY group (P<0.01) and normal control group (P<0.01). The levels of TNF-α and IFN-γ in the PD model group were 0.36 ∓ 0.11 ng/L and 0.83 ∓ 0.25 ng/L, respectively, also higher than those in the other two groups (P<0.01 or 0.001). But BSHXY group and the normal control group showed no significant differences in the levels of NO, TNF-α and IFN-γ (P>0.05).
CONCLUSIONThe therapeutic effect of BSHXY on PD is partially attributed to decreased content of NO, TNF-α and IFN-γ in the brain.
