Expression of ghrelin and its receptor GHS-R in the hypothalamus and gastrointestinal tract in rats with chronic renal failure.
- Author:
Rong-guo FU
1
;
Hao-zheng YUAN
;
Li WANG
;
Heng GE
;
Jun ZHANG
;
Qi-lan NING
;
Fu-jun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gastrointestinal Tract; metabolism; Ghrelin; genetics; metabolism; Hypothalamus; metabolism; Kidney Failure, Chronic; metabolism; Male; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors, Ghrelin; genetics; metabolism
- From: Journal of Southern Medical University 2011;31(1):96-99
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of ghrelin and its receptor, growth hormone secretagogue receptor (GHS-R), in the hypothalamus and gastrointestinal tract in rats with chronic renal failure (CRF) and explore their relationship with the disorder of gastrointestinal tract motility.
METHODSSD rats were randomly divided into sham-operated group (n=8) and CRF group (n=16), and in the latter group, the rats were subjected to 5/6 nephrectomy to induce CRF. Real-time PCR and immunohistochemical staining were used to detect the distribution of mRNA and protein of ghrelin and GHS-R in the gastric fundus, duodenum, and hypothalamus.
RESULTSThe rats in the CRF group showed a significantly higher expression of ghrelin mRNA and protein in the gastric fundus but a lower expression in the hypothalamus than those in the sham-operated group (P<0.01), but the expression in the duodenum was similar between the two groups (P>0.05). The expression of GHS-R mRNA and protein in the gastric fundus was significantly higher in the CRF group than in the sham-operated group (P<0.01), while in the hypothalamus and duodenum, the expression was significantly lower in the CRF group (P<0.01).
CONCLUSIONThe different distribution patterns of ghrelin and GHS-R in the tissues may be an important pathological basis of gastrointestinal motility disorder in CRF.
