Oxidation status and γ-glutamylcysteine synthetase expression in the kidney of MRL/lpr lupus mice.
- Author:
Rui-lin CHEN
1
;
Yi TAO
;
Bei-ping ZHANG
;
Zhi-fen LV
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Glutamate-Cysteine Ligase; genetics; metabolism; Glutathione; metabolism; Kidney; enzymology; metabolism; Lupus Nephritis; enzymology; metabolism; Malondialdehyde; metabolism; Mice; Mice, Inbred MRL lpr; Oxidative Stress; physiology; RNA, Messenger; genetics; metabolism
- From: Journal of Southern Medical University 2011;31(1):138-140
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE[corrected] To investigate the role of glutathione (GSH) and γ-glutamylcysteine synthetase (γ-GCS) in lupus nephritis.
METHODSSpectrophotometry was used to measure the oxidative/anti-oxidative indices including malonyldialdehyed (MDA) and GSH in the kidney of MRL/lpr lupus mice. Quantitative PCR and Western blotting were used to detected the expression of γ-GCS.
RESULTSThe level of GSH was lowered whereas the level of MDA increased significantly in the kidney tissue of MRL/lpr lupus mice as compared with that in normal control mice. The expression of γ-GCS mRNA and protein was significantly decreased in MRL/lpr lupus mice (P<0.05).
CONCLUSIONMRL/lpr lupus mice have abnormal oxidative stress in the kidney tissue, where the expression of γ-GCS decreased to lead to reduced GSH production, damaged antioxidative capacity, and eventually exacerbation of oxidative damage in the kidney.
