Effect of bone-marrow mesenchymal stem cells on the immune function of aging rats.
- Author:
Zhi-hong WANG
1
;
Kun-yuan GUO
;
Yu-lian YANG
;
Kun LIU
;
Jie LI
;
Xue-yun ZHOU
;
Qing-mei HE
Author Information
- Publication Type:Journal Article
- MeSH: Aging; immunology; Animals; Bone Marrow Cells; cytology; Female; Galactose; adverse effects; Interleukin-10; blood; Interleukin-2; blood; Male; Mesenchymal Stem Cell Transplantation; Random Allocation; Rats; Rats, Sprague-Dawley; Spleen; immunology; T-Lymphocytes; immunology
- From: Journal of Southern Medical University 2011;31(1):146-150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of transplantation of bone-marrow mesenchymal stem cells (MSCs) on the immune functions of aging rats.
METHODSHealthy SD rats were randomized into normal control, aging model group and MSCs group. The aging model was established by daily subcutaneous injection of D-galactose for 4 consecutive months. MSCs were isolated from the bone marrow of adult SD rats and injected (3×10(6) MSCs) in rats in the MSCs group via the tail vein once a week for 4 weeks. The spleen index, activity of T lymphocytes and the levels of IL-2 and IL-10 in spleen were measured, and the pathological changes of the spleen were observed after the treatments.
RESULTSMSCs transplantation enhanced the cellular immune function of aging rats manifested by obviously increased spleen index, activity of T lymphocyte and the level of IL-2, and lowered level of IL-10 in the spleen. The rats in the aging model group showed serious spleen injury, which was obviously lessened by MSCs injection.
CONCLUSIONMSCs transplantation can improve the cellular immune function of aging rats and ameliorate spleen injury induced by D-galactose.
