Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro.
- Author:
Xingjun YAO
1
;
Wei LI
;
Yuehua WANG
;
Zhengbin DING
;
Wenzhong HOU
;
Minmin ZENG
;
Guangce DENG
;
Jinzhu ZHANG
;
Hongfa YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Brain Neoplasms; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Female; Glioma; genetics; metabolism; pathology; Humans; Male; MicroRNAs; genetics; metabolism; Middle Aged; Real-Time Polymerase Chain Reaction; Young Adult
- From: Journal of Southern Medical University 2012;32(2):198-201
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro.
METHODSThe expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR. miR-20a mimics were synthesized and transfected into U251 cells via liposome, and the cell proliferation were detected using MTT assay and flow cytometry.
RESULTSThe glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues (P=0.035). The expression level of miR-20a was higher in high-grade than in low-grade gliomas. miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells.
CONCLUSIONmiR-20a shows potent effect in promoting the growth of glioma cells, suggesting its important role in the pathogenesis of human glioma.
