Effect of small interfering RNA targeting p63 on the proliferation and invasiveness of human cholangiocarcinoma cells in vitro.
- Author:
Zhan LIU
1
;
Mingliang ZHANG
;
Yini LI
;
Yu ZHANG
;
Zhoujun SHE
Author Information
- Publication Type:Journal Article
- MeSH: Bile Duct Neoplasms; genetics; pathology; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cholangiocarcinoma; genetics; pathology; Humans; Membrane Proteins; genetics; metabolism; Neoplasm Invasiveness; RNA, Small Interfering; genetics; Transfection
- From: Journal of Southern Medical University 2012;32(2):207-210
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a small interfering RNA (siRNA) vector targeting p63 and observe its effect on the proliferation and invasiveness of human cholangiocarcinoma cells in vitro.
METHODSReal-time PCR was used to examine the expression of p63 in human cholangiocarcinoma QBC939 cells. The recombinant lentivirus shRNA-p63 vector was constructed and transfected into QBC939 cells via Lipofectamine 2000 to establish a cholangiocarcinoma cell line with stable expression of siRNA-p63. The interfering efficiency of the siRNA targeting p63 was assessed using Western blotting. MTT and soft agar colony formation assays were used to evaluate the changes in the cell proliferation, and Boyden test was employed to observe the cell invasiveness after the transfection.
RESULTSQBC939 cells showed a high expression of p63. The recombinant lentivirus shRNA-p63 vector was successfully constructed as verified by sequencing. Transfection with the vector significantly suppressed the proliferation and invasiveness of QBC939 cells.
CONCLUSIONDown-regulation of p63 can inhibit the proliferation and invasiveness of human cholangiocarcinoma QBC939 cells in vitro.
