Effects of heat stress on Per2 mRNA expression in the liver of rats.
- Author:
Guanghua LI
1
;
Hong LI
;
Qinghua ZHANG
;
Hongmei LIU
;
Hongpeng LIU
;
Osamu SHIDO
Author Information
- Publication Type:Journal Article
- MeSH: Adrenal Glands; Animals; Body Temperature; Body Weight; Heat-Shock Response; genetics; Liver; metabolism; Male; Organ Size; Period Circadian Proteins; genetics; metabolism; Pituitary Gland; RNA, Messenger; genetics; metabolism; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2012;32(2):215-217
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of acute heat stress on the day-night circadian gene Per2 mRNA expression in the liver of rats.
METHODSMale Wistar rats were randomly divided into two groups and exposed to heat at 32 degrees celsius; or to a room temperature at 24 degrees celsius; (control). After 7 days of heat exposure, the body temperature was measured by telemetry. The relative weight of the pituitary and adrenal glands was determined after the exposure, and liver Per2 mRNA expression level was detected using RT-PCR.
RESULTSAcute heat stress did not obviously affect body temperature or body weight of the rats. Seven days of heat exposure increased the relative weight of the pituitary and adrenal glands and significantly lowered Per2 mRNA expression level at night.
CONCLUSIONAcute heat stress can interfere with the day-night circadian gene Per2 mRNA expression in rats.
