Role of centromere protein H in human gastric cancer cell proliferation.
- Author:
Tianyi QUAN
1
;
Benfu HE
;
Tiejian LIU
;
Weiming LI
;
Shangbiao WU
;
Qingping JIANG
;
Weiqiang LIU
;
Haibo LIU
;
Xuehu XU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Chromosomal Proteins, Non-Histone; genetics; metabolism; Humans; RNA, Messenger; genetics; metabolism; Real-Time Polymerase Chain Reaction; Stomach Neoplasms; metabolism; pathology
- From: Journal of Southern Medical University 2012;32(2):265-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of centromere protein H (CENP-H) in the proliferation of human gastric cancer cells.
METHODSRT-PCR and Western blot analysis were employed to examine the mRNA and protein expressions of CENP-H in 7 human gastric cancer cell lines and immortalized human gastric epithelial cells (GES-1). The cells were infected with the retrovirus vectors pMSCV-CENP-H or CENP-H-RNAi to establish stable cell lines with high CENP-H expression or CENP-H expression interference. MTT assay and colony formation assay were used to examine the changes in the cell proliferation after the infection.
RESULTSCENP-H was over-expressed in gastric cancer cell lines AGS, BGC823, SGC-7901, MKN45, HGC27, MGC-803 and MKN28 at both mRNA and protein levels. The established AGS/CENP-H cell line with increased CENP-H expression showed enhanced proliferative activity, while the cell line MGC-803/CENP-H-RNAi with CENP-H expression interference showed an obviously lowered proliferation ability.
CONCLUSIONCENP-H promotes the proliferation of human gastric cancer cells, suggesting its important role in the occurrence and development of gastric cancer.
