MiRNA-146a promotes proliferation and migration of rat vascular smooth muscle cells in vitro in a nuclear factor-κB-dependent manner.
- Author:
Wei XIONG
1
;
Shaohong DONG
;
Jianhui YUAN
;
Jianghua LI
;
Jianjun LIU
;
Xinyun XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Movement; drug effects; Cell Proliferation; drug effects; Male; MicroRNAs; antagonists & inhibitors; genetics; Muscle, Smooth, Vascular; cytology; drug effects; metabolism; Myocytes, Smooth Muscle; drug effects; metabolism; Primary Cell Culture; Proliferating Cell Nuclear Antigen; genetics; metabolism; Rats; Rats, Sprague-Dawley; Transcription Factor RelA; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2012;32(2):270-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE[corrected] To understand the role of miRNA-146a in the proliferation and migration of primarily cultured rat vascular smooth muscle cells (VSMCs) and investigate the mechanisms.
METHODSPrimarily cultured rat VSMCs were transfected with a synthesized miRNA-146 inhibitor, a scramble sequence or PBS via Lipofectamine2000. Cell counting kit 8 (CCK8) and transwell assay were employed to assess the proliferation and migration of the transfected cells, and the expressions of nuclear factor-κB p65 (NF-κBp65) and proliferation cell nuclear antigen (PCNA) were detected using Western blotting.
RESULTSA 48-h transfection of the VSMCs with miRNA-146 inhibitor caused significantly lowered miRNA-146a expression as compared with that in VSMCs transfected with the scramble sequence or PBS (P<0.01), resulting also in lowered proliferative and migration ability of the cells (P<0.01). The expression levels of NF-κBp65 and PCNA were remarkably lower in cells transfected with miRNA-146 inhibitor than in the cells in the other two groups (P<0.05).
CONCLUSIONmiRNA-146a is capable of promoting the proliferation and migration of rat VMSCs probably by enhancing the expression of NF-κBp65.
