Hsa-miR-654-5p regulates osteogenic differentiation of human bone marrow mesenchymal stem cells by repressing bone morphogenetic protein 2.
- Author:
Jun-Qiang WEI
1
;
Hua CHEN
;
Xiao-Fei ZHENG
;
Bo-Xun ZHANG
;
Yan WANG
;
Pei-Fu TANG
;
Fei SHE
;
Qing SONG
;
Tan-Shi LI
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Cells; cytology; Bone Morphogenetic Protein 2; antagonists & inhibitors; genetics; metabolism; Cell Differentiation; drug effects; Cells, Cultured; Gene Expression Regulation; Humans; Mesenchymal Stromal Cells; cytology; MicroRNAs; genetics; metabolism; Osteoblasts; cytology; Osteogenesis; genetics; RNA, Messenger; genetics; metabolism; Transfection
- From: Journal of Southern Medical University 2012;32(3):291-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of hsa-miR-654-5p in repressing bone morphogenetic protein 2 (BMP2) mRNA and protein in human bone marrow mesenchymal stem cells (hBMSCs), and explore its regulatory role in osteogenic differentiation of hBMSCs.
METHODShBMSCs in the 4th passage were cultured for 16 h and transfected with hsa-miR-654-5p followed by further culture for 48 h. qRT-PCR and Western blotting were performed to detect the expressions of BMP2 mRNA and protein. Dual-luciferase?reporter gene assay was employed to examine the repression of the BMP2 gene.
RESULTSBMP2 mRNA and protein expressions were significantly down-regulated in hBMSCs with hsa-miR-654-5p overexpression. Dualluci-ferase reporter gene assay indicated that the predicted target site of BMP2 was repressed directly by hsa-miR-654-5p, but this repression did not occur at the mutant predicted target site of BMP2.
CONCLUSIONhsa-miR-654-5p can directly repress the mRNA and protein expressions of BMP2 by binding to a specific target site. The changes in hsa-miR-654-5p can play an important role in osteogenic differentiation regulation of hBMSCs.
