Mechanisms of Chansu Injection in reversing multidrug resistance of HL60/ADM cells.
- Author:
Xin XIONG
1
;
Yuan-da ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Amphibian Venoms; pharmacology; Bufanolides; pharmacology; Doxorubicin; pharmacology; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; HL-60 Cells; Humans
- From: Journal of Southern Medical University 2012;32(3):337-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanisms underlying the effect of Chansu Injection (CHS) in reversing multi-drug resistance (MDR) of HL60/ADM cells.
METHODSMTT assay was used to investigate the effect of CHS on adramycin (ADM) sensitivity of HL-60/ADM cells. Flow cytometry was used to observe the effect of CHS on the cell cycle of HL60/ADM cell. The expressions of NF-κB, MRP, GST-π, and iNOS were detected by immunocytochemistry.
RESULTSTreatment with CHS lowered the IC(50) of ADM in HL60/ADM cells from 34.1971 µmol/L to 17.4393 µmol/L, and caused an increase in G(0)/G1 and G(2)/M phase cells with decreased S phase cells. CHS decreased the expressions of MRP mRNA and GST-π and MRP proteins but increased the expressions of iNOS and NF-κB proteins in the cells.
CONCLUSIONCHS can partly reverse MDR in HL60/ADM cells possibly by down-regulating MRP and GST-π, up-regulating NF-κB and iNOS, and promoting cell apoptosis, thereby increase ADM sensitivity of HL-60/ADM cells.
