Effect of arctiin on mouse podocyte epithelial-mesenchymal transition induced by advanced oxidation protein products.
- Author:
Jun ZHANG
1
;
Ting-Ting GUO
;
Lei YANG
;
Qing-Sheng DU
;
Jie HUA
;
Rong-Zhi LIU
;
Xun TANG
Author Information
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Advanced Oxidation Protein Products; adverse effects; Animals; Cell Line; Endoplasmic Reticulum Stress; drug effects; Epithelial-Mesenchymal Transition; drug effects; Furans; pharmacology; Glucosides; pharmacology; Heat-Shock Proteins; metabolism; Mice; Podocytes; metabolism; pathology; Transcription Factor CHOP; metabolism
- From: Journal of Southern Medical University 2012;32(3):379-382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition (EMT) induced by advanced oxidation protein products (AOPP).
METHODSMouse podocytes were stimulated by 200 µg/ml AOPP for 24 h in the presence of 50, 100, 200, and 400 µmol/L arctiin. The expressions of α-smooth muscle actin, Grp78 and CHOP were detected using Western blotting.
RESULTThe expressions of α-SMA, Grp78 and CHOP were inhibited by arctiin, showing a dose-dependent effect within a given range of arctiin concentration.
CONCLUSIONAOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes, and arctiin can decrease EMT by alleviating the stress. This finding sheds light on a new scope of research of renal fibrosis.
