Application of genome-wide microarray for screening genes related to peritoneal metastasis of colorectal cancer.
- Author:
Feng LIU
1
;
Jiu-Bing GUO
;
Zhi-Yong SHEN
;
Ting-Yu MU
;
Peng-Ke ZHI
;
Guo-Xin LI
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; secondary; Adenocarcinoma, Mucinous; genetics; secondary; Adult; Aged; Calcium-Binding Proteins; genetics; metabolism; Colorectal Neoplasms; genetics; pathology; Female; Gene Expression Profiling; Genome-Wide Association Study; Humans; Male; Middle Aged; Neoplasm Proteins; genetics; metabolism; Oligonucleotide Array Sequence Analysis; methods; Peritoneal Neoplasms; genetics; secondary; Peroxiredoxins; genetics; metabolism; Secretory Leukocyte Peptidase Inhibitor; genetics; metabolism
- From: Journal of Southern Medical University 2012;32(3):400-403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen genes related to peritoneal metastasis of colorectal cancer.
METHODSSpecimens of primary cancer and normal mucosa tissues were collected from 3 patients with peritoneal metastasis of colorectal cancer. The total RNA were extracted and inversely transcribed into cDNA to synthesize aRNA using in vitro RNA synthesis. The synthesized aRNA, after labeling with Cy3, were hybridized with the whole human genome oligo microarray. The Empirical Bayes method was used to screen the differentially expressed genes, followed by confirmation of the selected genes by semi-quantitative RT-PCR.
RESULTSWith a threshold of P≤0.05, a total of 105 differentially expressed genes were identified in primary cancer lesions, including 42 up-regulated and 63 down-regulated genes. Three of the up-regulated genes (S100P, PRDX1 and SLPI) were selected and confirmed by RT-PCR, which yielded results consistent with those from gene microarray.
CONCLUSIONGene microarray technique can provide valuable clues for locating the tumor markers of peritoneal metastasis in colorectal cancer patients.
