Adipose tissue sample preparation for scanning electron microscopy.
- Author:
Fang CUI
1
;
Chao LIU
;
Jian-Min YANG
;
Li WANG
Author Information
1. Laboratory Center of Electron Microscopy, Hebei Medical University, Shijiazhuang 050017, China. fanghua1980@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adipose Tissue;
ultrastructure;
Animals;
Female;
Humans;
Microscopy, Electron, Scanning;
Rats;
Rats, Sprague-Dawley;
Specimen Handling;
methods
- From:
Journal of Southern Medical University
2012;32(3):435-436
- CountryChina
- Language:Chinese
-
Abstract:
Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy (SEM). We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids, phospholipids protein, and hence the membrane structure. Also by extending the dehydration time to fully replace the organic solvents, we achieved satisfactory results for SEM of adipose samples.
