Differences in fecal Bifidobacterium species between patients with type 2 diabetes and healthy individuals.
- Author:
Xiaojin XU
1
;
Hongxiang HUI
;
Dehong CAI
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Bifidobacterium; classification; isolation & purification; Case-Control Studies; Diabetes Mellitus, Type 2; microbiology; Feces; microbiology; Female; Humans; Intestines; microbiology; Male; Middle Aged; Real-Time Polymerase Chain Reaction
- From: Journal of Southern Medical University 2012;32(4):531-564
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the changes in fecal Bifidobacterium species in patients with type 2 diabetes in comparison with healthy individuals.
METHODSThe bacterial DNA were extracted from the fecal samples from 50 type 2 diabetic patients and 30 healthy individuals. Real-time quantitative PCR was employed to determine the copy numbers of the bacteria in the fecal samples using 16S rRNA-targeted genus- and species-specific PCR primers for a selected group of fecal Bifidobacterium species including total Bifidobacterium, B.longum, B.breve, B.adolescent, and B. infantis.
RESULTSThe diabetic group had significantly lower copy numbers of total Bifidobacterium and B.adolescent compared to the healthy individuals (P<0.05).
CONCLUSIONType 2 diabetic patients have a lowered number of Bifidobacterium species in the gut microflora.
