Pseudomonas aeruginosa vaccine inhibits the proliferation of human nasopharyngeal cancer cells in vitro.
- Author:
Jian WANG
1
;
Dehua WU
;
Longhua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cancer Vaccines; immunology; Cell Line, Tumor; Cell Proliferation; drug effects; Humans; Nasopharyngeal Neoplasms; pathology; Pseudomonas aeruginosa; immunology
- From: Journal of Southern Medical University 2012;32(4):544-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitory effects of Pseudomonas aeruginosa vaccine (PA-MSHA) on the proliferation of human nasopharyngeal cancer cells and explore the possible mechanism.
METHODSMTT assay was used to determine the cell growth of human nasopharyngeal cancer cell line 5-8F and 6-10B in vitro treated with the vaccine. The cell cycle distribution of the cells was detected by flow cytometry, and the expression levels of apoptosis and cycle-related proteins were evaluated by Western blotting.
RESULTSPA-MSHA treatment significantly suppressed the proliferation of 5-8F and 6-10B cells in a time- and concentration-dependent manner compared with the control group (P<0.05). The cells with PA-MSHA treatment exhibited a decreased percentage of cells entering S phase and a corresponding increase in G(1) phase cells in FACS analysis. The expression of cyclin D(1), CDK4, and CDK6 was significantly up-regulated, Bax protein up-regulated, and the anti-apoptosis protein Bcl-2 down-regulated in PA-MSHA-treated cells.
CONCLUSIONPA-MSHA can suppress the proliferation of nasopharyngeal carcinoma cell in vitro by affecting the cell cycle and promoting cell apoptosis.
