Effect of schizandrin B on H(2)O(2)-induced apoptosis of human hepatocytes in vitro: role of Fas pathway.
- Author:
Jing CAI
1
;
Qiaobing HUANG
;
Debiao CHI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 8; metabolism; Cell Line; Cyclooctanes; pharmacology; Fas-Associated Death Domain Protein; metabolism; Flow Cytometry; Hepatocytes; drug effects; metabolism; Humans; Hydrogen Peroxide; adverse effects; Lignans; pharmacology; Polycyclic Compounds; pharmacology; Signal Transduction; fas Receptor; metabolism
- From: Journal of Southern Medical University 2012;32(4):583-592
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Fas pathway in H(2)O(2)-induced apoptosis of L02 human hepatocytes and the effect of schisandrin B on Fas pathway.
METHODSReal-time quantitative PCR was used to detect the expressions of FAS, fas associated death domain protein (FADD) and caspase-8 mRNA in L02 cells exposed to H(2)O(2). Flow cytometry was employed to assess the cell apoptosis. ELISA, Western blotting and spectrophotometric assay were performed to determine the expressions of FAS protein, FADD protein and caspase-8 activity.
RESULTSWithin the dose range of 5-15 mol/L, schisandrin B dose-dependently inhibited FAS and FADD expressions and caspase-8 activation.
CONCLUSIONSchisandrin B can partially inhibit H(2)O(2)-induced L02 cell apoptosis possibly by affecting the FAS-FADD-caspase-8 pathway.
