Susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to infection with bat Japanese encephalitis virus isolates.
- Author:
Shan LIU
1
;
Qionghua ZHANG
;
Junhua ZHOU
;
Shouyi YU
;
Xueli ZHENG
;
Qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aedes; virology; Animals; Chiroptera; virology; Culex; virology; Disease Susceptibility; Encephalitis Virus, Japanese; isolation & purification
- From: Journal of Southern Medical University 2012;32(4):515-518
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to oral infection with bat Japanese encephalitis virus isolates (GD1 and HN2 strains).
METHODSAedes albopictus and Culex pipiens quinquefasciatus were infected orally by GD1 and HN2 strains of bat Japanese encephalitis virus. TaqMan real-time PCR was used to detect the virus and monitor the changes in the viral loads in Aedes albopictus and Culex pipiens quinquefasciatus at a 2-day interval, starting from 4 days till 20 days after the infection.
RESULTSThe infected Aedes albopictus and Culex pipiens quinquefasciatus were found positive for the Japanese encephalitis virus from day 4 to day 20. Both Aedes albopictus and Culex pipiens quinquefasciatus were susceptible to infection by GD1 and HN2 strains, but the latter showed a greater susceptibility. The HN2 strain virus appeared to have a greater virulence than the GD1 strain.
CONCLUSIONAedes albopictus and Culex pipiens quinquefasciatus can carry GD1 and HN2 strains of bat Japanese encephalitis virus isolates.
