Microinjection of NMDA-type glutamate receptor agonist NMDA and antagonist D-AP-5 into the central nucleus of the amygdale alters water intake rather than food intake.
- Author:
Junbao YAN
1
;
Jianqun YAN
;
Jinrong LI
;
Ke CHEN
;
Huiling SUN
;
Yuan ZHANG
;
Xiaolin ZHAO
;
Bo SUN
;
Shiru ZHAO
;
Lin SONG
;
Xiaojing WEI
Author Information
- Publication Type:Journal Article
- MeSH: 2-Amino-5-phosphonovalerate; pharmacology; Amygdala; drug effects; Animals; Drinking; drug effects; Eating; drug effects; Excitatory Amino Acid Agonists; pharmacology; Excitatory Amino Acid Antagonists; pharmacology; Injections, Intraventricular; Male; N-Methylaspartate; pharmacology; Rats; Rats, Sprague-Dawley; Receptors, N-Methyl-D-Aspartate; agonists; antagonists & inhibitors
- From: Journal of Southern Medical University 2012;32(5):595-600
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the role of N-Methyl-D-aspartic acid (NMDA)-type glutamate receptors in the central nucleus of the amygdale (CeA) in food and water intake.
METHODSMale Sprague-Dawley rats with stainless steel cannulae implanted unilaterally into the CeA were used. The prototypic NMDA receptor agonist NMDA, or the selective NMDA receptor antagonist D(-)-2-amino-5-phosphonopentanoic acid (D-AP-5) was microinjected into the CeA of satiated and euhydrated rats.
RESULTSIntra-CeA injection of 8.50, 17.00, or 34.00 nmol NMDA did not alter food intake but significantly increased water intake 0-1 h after the injection (F(3,32)=3.191, P=0.037) independent of food intake. Without affecting the food intake, injection of 6.34, 12.70, or 25.40 nmol D-AP-5 into the CeA significantly decreased water intake 0-1 h after the injection (F(3,28)=3.118, P=0.042) independent of food intake.
CONCLUSIONNMDA receptors in the CeA may participate in the control of water intake rather than food intake.
