miR-124 suppresses cell proliferation and invasion in gastric carcinoma and its mechanism.
- Author:
Li-ming XIE
1
;
Rong-fang HE
;
Zhi-wei ZHANG
;
Yun-yun TANG
;
Zhao-yang LUO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Humans; Luciferases; genetics; metabolism; MicroRNAs; genetics; Neoplasm Invasiveness; Phosphotransferases (Alcohol Group Acceptor); genetics; metabolism; RNA, Small Interfering; genetics; Recombinant Proteins; genetics; metabolism; Stomach Neoplasms; metabolism; pathology; Transfection
- From: Chinese Journal of Oncology 2013;35(7):497-500
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanism of miR-124 suppressing the proliferation and invasion of gastric cancer cells.
METHODSSPHK1 3'UTR-luciferase vector was constructed and luciferase reporter gene assay was employed to examine the effect of miR-124 on luciferase activity. Human gastric cancer MGC-803 cells were transfected with miR-124 mimics, and then Western blot was performed to detect the expression of SPHK1 protein.
RESULTSLuciferase reporter vector system confirmed that SPHK1 was a target gene of miR-124. Western blot showed that the expression of SPHK1 protein was inhibited by miR-124. After transfection of miR-124 mimics or SPHK1 siRNA for 12 h, 24 h and 48 h, respectively, MTT assay showed that the A values of the three groups were significantly different (P < 0.05), and it was in a time-dependent manner. After transfection of miR-124 mimics or SPHK1 siRNA for 24 h, transwell invasion assay showed that the number of transmembrane cells was 54.6 ± 8.3 in the SPHK1 siRNA group and 47.8 ± 6.6 in the miR-124 mimics group, both were significantly lower than 100.6 ± 11.3 of the control group (P < 0.05), indicating that SPHK1 siRNA can slow down the invasion of MGC-803 cells.
CONCLUSIONmiR-124 can suppress the cell proliferation and invasion by targeting SPHK1 in gastric carcinoma.
