Immunophenotyping of leukemic stem cells and chromosome karyotype characteristics in Uyghur leukemia pediatric patients.
- Author:
Nuriding HAILIQIGULI
1
;
Mei YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Antigens, CD; metabolism; Antigens, CD19; metabolism; Child; China; ethnology; Diploidy; Humans; Immunophenotyping; Interleukin-3 Receptor alpha Subunit; metabolism; Karyotyping; Leukemia, Myeloid, Acute; drug therapy; genetics; immunology; pathology; Neoplastic Stem Cells; immunology; Precursor Cell Lymphoblastic Leukemia-Lymphoma; drug therapy; genetics; immunology; pathology; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; drug therapy; genetics; immunology; pathology; Remission Induction; Tetraspanin-29; metabolism; Thy-1 Antigens; metabolism; Translocation, Genetic
- From: Chinese Journal of Oncology 2013;35(7):501-504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the immunophenotype and chromosome karyotype characteristics of leukemic stem cells (LSC) in Uyghur leukemia pediatric patients.
METHODSThe morphological features of LSC in culture in vitro was observed by flow cytometry. The immunophenotype was assessed by detective flow cytometry. The chromosome karyotype was analyzed by R-banding technique.
RESULTSThe LSC showed suspended floating colonies growing in the culture medium, and grew well and proliferated constantly in culture over 8 months. Among the 13 children with AML, there were 10 CD34(+)CD38(-)CD123(+) and CD33(+) cases, 10 CD44(+) cases, 10 CD96(+) cases, and 5 CD90(+) cases. Among the 13 children with B-ALL, there were 6 CD34(+)CD20(-)CD19(+) cases, 7 CD9(+) cases, and 5 CD123(+) cases. Among the 9 children with acute T lymphoblastic leukemia (T-ALL), there were 5 CD34(+)CD7(-) and CD90(+) cases, and 4 CD123(+) cases. Among the 13 cases of AML, 5 cases showed chromosome translocation t(15;17), one case chromosome translocation t(8;21), and 7 cases showed no chromosome karyotype abnormality. Among the 22 ALL cases, there were chromosome translocation t(12;21) in 1 case, t(9;22) in 3 case, hyperdiploid in 2 cases, and 16 cases without karyotype abnormalities. Twenty-nine children received induction remission therapy. Among them, 12 died, including 9 CD96(-)positive cases and 3 CD96(-)negative cases, with a statistically significant difference (P < 0.05).
CONCLUSIONSThe LSC of Uyghur leukemia pediatric patients in Xinjiang express CD9 and CD19 in ALL, and express CD123 and CD90 simultaneously in ALL and AML. The expression of CD96 is one of factors of poor prognosis.
