Aberrant methylation of hMLH1 gene promoter in papillary thyroid cancer and its clinical significance.
- Author:
Xiao-xiao LU
1
;
Ming-hua GE
2
;
Zhi-qiang LING
;
Si-si HU
;
Jing XU
;
Chuan-ming ZHENG
;
Zhuo TAN
;
Chao CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; genetics; metabolism; Adolescent; Adult; Age Factors; Aged; Carcinoma; genetics; metabolism; pathology; Carcinoma, Papillary; DNA Methylation; Female; Gene Expression Regulation, Neoplastic; Humans; Lymphatic Metastasis; Male; Middle Aged; MutL Protein Homolog 1; Neoplasm Invasiveness; Neoplasm Staging; Nuclear Proteins; genetics; metabolism; Promoter Regions, Genetic; genetics; Thyroid Neoplasms; genetics; metabolism; pathology; Tumor Burden; Young Adult
- From: Chinese Journal of Oncology 2013;35(11):833-836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the aberrant promoter methylation of hMLH1 gene promoter and its clinical significance in papillary thyroid cancer (PTC).
METHODSmethylation of hMLH1 gene promoter in the cancer tissue and matched tumor-adjacent normal tissue of 152 PTC patients were detected by real-time methylation specific PCR (qMSP). The relationship between the methylation of hMLH1 gene promoter and clinicopathological features was analyzed.
RESULTSThe methylation rate of hMLH1 gene promoter in cancer tissues was 37.5% (57/152), of which 33 cases were totally methylated and 24 cases were partially methylated. The methylation rate of adjacent normal tissues was 5.3% (8/152)(all were partially methylated). The methylation rate of PTC tissues was significantly higher than that in the tumor-adjacent normal tissue (P < 0.01). The promoter methylation of hMLH1 gene in PTC was significantly correlated with age, size and number of the primary lesion, local invasion, T stage and lymph node metastasis (P < 0.05) , but not correlated with gender and clinical stage (P > 0.05).
CONCLUSIONPromoter methylation of hMLH1 gene is a common molecular event in PTC tissue, and it is significantly correlated with the progression of PTC.
