Cloning of yeast Pichia pastoris coded synthesis of HBsAg DNA recombinant
- Author:
Anh Thuy Ngo
- Publication Type:Journal Article
- Keywords:
virus
- MeSH:
Cloning, Organism;
Hepatitis B Surface Antigens;
DNA, Recombinant
- From:Journal of Preventive Medicine
2001;11(2):40-45
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The DNA fragment in HBV genome, which codes for HBsAg, was reconstructed from oligodeoxynucleotides using PCR method. After checking for correct nucleotid sequences by DNA sequencing, the DNA fragment coding for HBsAg was ligated into the plasmid vector pPICZA. Restricted enzymes EcoR I and Noti were utilized to cut DNA - plasmid and positive clones containing HBsAg-coding DNA-plasmid were selected by agarose gel electrophoresis
