Research on genotyping of methicillin-resistant Staphylococcus aureus in China
10.3760/cma.j.issn.0254-6450.2010.03.017
- VernacularTitle:中国15个地区分离的耐甲氧西林金黄色葡萄球菌基因分型研究
- Author:
En-Hua SHEN
1
;
Li-Hong WANG
;
Hui WANG
;
Hong-Li SUN
;
Min-Jun CHEN
;
Jing YUAN
;
Yan-Kuan WANG
Author Information
1. 吉林大学第四医院
- Keywords:
Methicillin-resistant Staphylococcus aureus;
Multilocus sequence typing;
Staphylococcal cassette chromosome mec;
Staphylococcus protein A typing
- From:
Chinese Journal of Epidemiology
2010;31(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the source and genetic background of methicillin-resistant Staphylococcus aureus in the year of 2006,in China. Methods From January to December 2006,a total number of 302 consecutive and non-repetitive methicillin-resistant Staphylococcus aureus were collected from 17 Teaching hospitals in 15 areas. Genotypes of SCCmec were determined by multiplex PCR and multilocus sequence typing (MLST) was used to type the house-keeping genes. The implementation of the spa typing method was straightforward,and the results obtained were reproducible,unambiguous,and easily interpreted. Results All areas but Dalian harbored SCCmec Ⅲ while Dalian harbored SCCmec Ⅱ most. There were two strains in Guangzhou,harboring SCCmec Ⅳ. There were four strains of sequence type(ST),with ST239 accounted for 46.7% and ST5 accounted for 44.4%. ST59 accounted for 6.7% and ST88 accounted for 2.2%. There were fourteen strains of Spa typing,with t30 accounted for 52.6% ; t37 accounted for 27.2% ; t2 accounted for 12.9% ; t632 accounted for 2.3% ; t437 accounted for 1.3% ; t570,t601 accounted for 0.7% ; t377,t459,t796,t899,t1152,t2649 accounted for 0.3% ; no-typing accounted for 0.3%,respectively,pvl gene was not detected. Conclusion The main clone strains were ST239-MRSA-SCCmec Ⅲ-t30,ST5-MRSA-SCCmec Ⅱ-t2,with unique geographic distributions across the whole nation.
