The establishment of a rapid fluorescent focus inhibition test for testing rabies virus neutralizing antibody
10.3760/cma.j.issn.0254-6450.2010.04.018
- VernacularTitle:狂犬病毒中和抗体检测快速荧光灶抑制试验的建立
- Author:
Peng-Cheng YU
1
;
Xin-Jun LV
;
Xin-Xin SHEN
;
Lei CAO
;
Xin-Xiong ZHENG
;
Hu SHAN
;
Qing TANG
Author Information
1. 青岛农业大学
- Keywords:
Rabies virus;
Neutralizing antibody;
Fluorescent inhibition test;
Titer
- From:
Chinese Journal of Epidemiology
2010;31(4):438-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody.CVS-11 was used as the standard challenge virus,and three generations prepared for the establishment of the virus library.Methods International standard for rabies immunoglobulin was used as the reference serum.RFFIT test was established under consulting the protocol of Institute of Pasteur,and its specificity,stability and reproducibility were validated.Results We established the RFFIT which showed both good specificity ( 100% ) and reproducibility (P＞0.5).Conclusion The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.
