Jiaotai Pill enhances insulin signaling through phosphatidylinositol 3-kinase pathway in skeletal muscle of diabetic rats.
- Author:
Hui DONG
1
;
Jian-hong WANG
;
Fu-er LU
;
Li-jun XU
;
Yan-lin GONG
;
Xin ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Body Weight; drug effects; Diabetes Mellitus, Experimental; drug therapy; enzymology; Drugs, Chinese Herbal; pharmacology; therapeutic use; Glucose Tolerance Test; Glucose Transporter Type 4; metabolism; Homeostasis; drug effects; Insulin; metabolism; Insulin Receptor Substrate Proteins; metabolism; Insulin Resistance; Male; Muscle, Skeletal; drug effects; enzymology; metabolism; pathology; Phosphatidylinositol 3-Kinases; metabolism; Phosphorylation; Phosphotyrosine; metabolism; Protein Subunits; metabolism; Rats; Rats, Wistar; Receptor, Insulin; metabolism; Signal Transduction; drug effects
- From: Chinese journal of integrative medicine 2013;19(9):668-674
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of Jiaotai Pill (, JTP) at different constitutional proportions on insulin signaling through phosphatidylinositol 3-kinase (PI3K) pathway in the skeletal muscle of diabetic rats.
METHODSThe rat model of type 2 diabetes mellitus (T2DM) was established by intravenous injection of a small dose of streptozotoein plus high fat diet feeding. JTP at the same dosage of cinnamon and the increasing dosage of Coptis chinensis was administered to diabetic rats for nine weeks respectively. Plasma glucose and insulin levels were assayed. The expressions of proteins were determined by Western blot method.
RESULTSAll the three formulations of JTP decreased plasma glucose and fasting insulin levels as well as increased the protein expressions of insulin receptor β (InsRβ) subunit, insulin receptor substrate-1 (IRS-1), PI3K p85 subunit and glucose transporter 4 (GLUT4) in skeletal muscle. Meanwhile, JTP increased the tyrosine phosphorylation of InsRβ subunit and IRS-1, and reduced the serine phosphorylation of IRS-1 in skeletal muscle. Interestingly, the effect of JTP on improving insulin sensitivity was not dose-dependent. In contrast, JTP containing the least amount of Coptis chinensis exhibited the best effect.
CONCLUSIONJTP at different constitutional proportions attenuates the development of diabetes in a rat model of T2DM. The mechanism might be associated with enhancing insulin signaling through PI3K pathway in the skeletal muscle.
