Modified Xiaochaihu Decoction () prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway.
- Author:
Shu-kun ZHANG
1
;
Nai-qiang CUI
;
Yu-zhen ZHUO
;
Di-hua LI
;
Jun-hong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Amylases; blood; Animals; Base Sequence; Blood Glucose; metabolism; Body Weight; drug effects; Chronic Disease; DNA Primers; Disease Progression; Drugs, Chinese Herbal; therapeutic use; Lipase; blood; Male; Pancreatitis; drug therapy; metabolism; pathology; RNA, Messenger; genetics; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction; Signal Transduction; drug effects; Smad Proteins; genetics; metabolism; Transforming Growth Factor beta1; metabolism
- From: Chinese journal of integrative medicine 2013;19(12):935-939
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effect of modified Xiaochaihu Decoction (, MXD) on transforming growth factor-β1/Sma- and Mad-related proteins (TGF-β1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride.
METHODSThirty healthy male Wistar rats were randomly divided into the normal control group, CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC, 7 mg/kg of body weight) into the right caudal vein, and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight, starting from the day after CP induction. After 28-day treatment, the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then, rats were sacrificed, and pancreatic tissues were harvested for histological evaluation. In addition, the mRNA expression of TGF-β1, TGF-β1 type II receptor (TGFβRII), Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction.
RESULTSTreatment of CP with MXD improved the PABA recovery, decreased the histological lesion, and reduced the mRNA expression of TGF-β1, TGFβRII and Smad3 (P<0.05). However, MXD had no effect on Smad7 mRNA level.
CONCLUSIONSMXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-β1/Smads signaling pathway.
